Difference between revisions of "Part:BBa K1899006"
 
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<partinfo>BBa_K1899006 short</partinfo>
 
<partinfo>BBa_K1899006 short</partinfo>
  
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The construct aims at investigating any interference caused by lacl repressor on promoter <i>PhlFp</i>.
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This construct aims at investigating any interference caused by Lacl repressor on promoter <i>phlFp</i>.
  
 
===Results===
 
===Results===
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The fold change between construct A (pSB3K3-BBa_E0240-<i>phlFp</i>) and negative control (pSB3K3-BBa_E0240), and that of construct C (pSB3K3-BBa_J23101-B0032-C0012-B1006- <i>phlFp</i> -E0240) were both approximately 13.2. Since their RFU are similar, it indicated that there is no cross-talk between <i>lacp</i> and <i>phlFp</i> (<bbpart>(BBa_K1899004</bbpart>).  
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https://static.igem.org/mediawiki/parts/thumb/2/25/Comparison_of_the_Strength_of_tetR_and_PhlFp.jpeg/748px-Comparison_of_the_Strength_of_tetR_and_PhlFp.jpeg
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[[File:IGEM2016_HKUST_phlFK1899006.png|thumb|600px|center|<b>Fig 1. Comparison on fluorescence expression levels of construct A (BBa_<i>phlFp</i>-E0240) and construct C (BBa_J23101-B0032-C0012-B1006- <i>phlFp</i> -E0240)</b>. Negative control represents BBa_E0240. Characterization was done using <i>E. coli</i> strain JW0336. Cells were first precultured overnight and were subcultured to mid-log phase where GFP emission measurements were made using an EnVision® multilabel reader. This result was obtained by combining 3 characterization data obtained in 3 different days. Error bar present SD from 3 biological replicates.]]
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<b>Fig a) Comparison of Technical Triplicate Results of pSB3K3-BBa_J23101-B0032-C0012-B1006- PhlFp -E0240 and  pSB3K3-PhlFp - BBa_E0240</b>
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The fold change between <b>pSB3K3-PhlFp - BBa_E0240 and negative control (pSB3K3-BBa_E0240)</b> is 13.2, which is same as that of <b>pSB3K3-BBa_J23101-B0032-C0012-B1006- PhlFp -E0240</b>. Since their RFU are similar and almost the same, it proves that there is no cross-talk between <i>lacp</i> and <i>Phlfp</i>(<bbpart>(BBa_K1899004</bbpart>).  
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Latest revision as of 15:37, 18 October 2016


J23101-B0032-lacl-B1006- phlFp-GFP

This construct aims at investigating any interference caused by Lacl repressor on promoter phlFp.

Results

The fold change between construct A (pSB3K3-BBa_E0240-phlFp) and negative control (pSB3K3-BBa_E0240), and that of construct C (pSB3K3-BBa_J23101-B0032-C0012-B1006- phlFp -E0240) were both approximately 13.2. Since their RFU are similar, it indicated that there is no cross-talk between lacp and phlFp (BBa_K1899004).

Fig 1. Comparison on fluorescence expression levels of construct A (BBa_phlFp-E0240) and construct C (BBa_J23101-B0032-C0012-B1006- phlFp -E0240). Negative control represents BBa_E0240. Characterization was done using E. coli strain JW0336. Cells were first precultured overnight and were subcultured to mid-log phase where GFP emission measurements were made using an EnVision® multilabel reader. This result was obtained by combining 3 characterization data obtained in 3 different days. Error bar present SD from 3 biological replicates.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1209
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2007