Difference between revisions of "Part:BBa K1898150:Design"

 
 
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<partinfo>BBa_K1898150 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1898150 SequenceAndFeatures</partinfo>
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===Design Notes===
 
===Design Notes===
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We made sure that there was no Ecor1, Xbal, Spel1, and Pst1 cutting sites in the DNA.
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We performed one silent point mutation on the CH25H DNA to remove one internal cutting site. The stop codon in CH25H was removed as well.
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The following primers are designed and used to move the DNA synthesized by IDT into iGEM Biobrick:
  
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forward: 5' ATATgAATTCgCggCCg 3' (17)
  
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reverse: 5' ATATCTgCAgCggCC 3' (15)
  
 
===Source===
 
===Source===
  
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this DNA is synthesized by IDT.  
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This DNA is synthesized by IDT.  
  
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===References===
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Primers were synthesized by Tri-I biotech.

Latest revision as of 15:31, 18 October 2016


Strong promoter + Strong RBS + CH25H + 10x His tag + Double terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Design Notes

We performed one silent point mutation on the CH25H DNA to remove one internal cutting site. The stop codon in CH25H was removed as well.

The following primers are designed and used to move the DNA synthesized by IDT into iGEM Biobrick:

forward: 5' ATATgAATTCgCggCCg 3' (17)

reverse: 5' ATATCTgCAgCggCC 3' (15)

Source

This DNA is synthesized by IDT.

Primers were synthesized by Tri-I biotech.