Difference between revisions of "Part:BBa K1898550"
| (One intermediate revision by the same user not shown) | |||
| Line 3: | Line 3: | ||
<partinfo>BBa_K1898550 short</partinfo> | <partinfo>BBa_K1898550 short</partinfo> | ||
| − | Strong promoter and strong rbs (BBa_K880005) | + | Strong promoter and strong rbs (BBa_K880005) are used to maximize protein output. The ORF includes green fluorescence protein and a 10x Histidine tag for protein purification. BBa_B0015 consists of a double terminator to end transcription. |
This part can be used to test protein purification method or be used as a way to quantify protein expression level. | This part can be used to test protein purification method or be used as a way to quantify protein expression level. | ||
| Line 23: | Line 23: | ||
==synthesis report== | ==synthesis report== | ||
This construct is synthesized by IDT. The following sequence was sent to IDT to synthesize, and each feature is annotated as shown below: | This construct is synthesized by IDT. The following sequence was sent to IDT to synthesize, and each feature is annotated as shown below: | ||
| + | *Prefix and suffix are highlighted in orange | ||
| + | *BBa_K880005 is highlighted in red | ||
| + | *GFP is highlighted in light blue | ||
| + | *10x Histidine is highlighted in green | ||
| + | *B0015 is highlighted in dark blue | ||
https://static.igem.org/mediawiki/parts/3/36/Bpht_sequence.png | https://static.igem.org/mediawiki/parts/3/36/Bpht_sequence.png | ||
Latest revision as of 15:28, 18 October 2016
Strong promoter + Strong RBS + GFP + 10x Histidine tag + double terminator
Strong promoter and strong rbs (BBa_K880005) are used to maximize protein output. The ORF includes green fluorescence protein and a 10x Histidine tag for protein purification. BBa_B0015 consists of a double terminator to end transcription.
This part can be used to test protein purification method or be used as a way to quantify protein expression level.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 705
synthesis report
This construct is synthesized by IDT. The following sequence was sent to IDT to synthesize, and each feature is annotated as shown below:
- Prefix and suffix are highlighted in orange
- BBa_K880005 is highlighted in red
- GFP is highlighted in light blue
- 10x Histidine is highlighted in green
- B0015 is highlighted in dark blue
IDT report shows that the sequence is successfully synthesized:
