Difference between revisions of "Part:BBa K1898550"

 
 
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<partinfo>BBa_K1898550 short</partinfo>
 
<partinfo>BBa_K1898550 short</partinfo>
  
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BBa_K880005 is used to provide a strong promoter and strong rbs to maximize protein output. The ORF includes green fluorescence protein and a 10x Histidine tag for protein purification. BBa_B0015 consists of a double terminator to end transcription.
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Strong promoter and strong rbs (BBa_K880005) are used to maximize protein output. The ORF includes green fluorescence protein and a 10x Histidine tag for protein purification. BBa_B0015 consists of a double terminator to end transcription.
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This part can be used to test protein purification method or be used as a way to quantify protein expression level.  
  
  
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<partinfo>BBa_K1898550 parameters</partinfo>
 
<partinfo>BBa_K1898550 parameters</partinfo>
 
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==synthesis report==
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This construct is synthesized by IDT. The following sequence was sent to IDT to synthesize, and each feature is annotated as shown below:
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*Prefix and suffix are highlighted in orange
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*BBa_K880005 is highlighted in red
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*GFP is highlighted in light blue
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*10x Histidine is highlighted in green
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*B0015 is highlighted in dark blue
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https://static.igem.org/mediawiki/parts/3/36/Bpht_sequence.png
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IDT report shows that the sequence is successfully synthesized:
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https://static.igem.org/mediawiki/parts/0/0a/Bpht_idt_2.png
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https://static.igem.org/mediawiki/parts/8/88/Bpht_idt_1.png

Latest revision as of 15:28, 18 October 2016


Strong promoter + Strong RBS + GFP + 10x Histidine tag + double terminator

Strong promoter and strong rbs (BBa_K880005) are used to maximize protein output. The ORF includes green fluorescence protein and a 10x Histidine tag for protein purification. BBa_B0015 consists of a double terminator to end transcription.

This part can be used to test protein purification method or be used as a way to quantify protein expression level.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 705


synthesis report

This construct is synthesized by IDT. The following sequence was sent to IDT to synthesize, and each feature is annotated as shown below:

  • Prefix and suffix are highlighted in orange
  • BBa_K880005 is highlighted in red
  • GFP is highlighted in light blue
  • 10x Histidine is highlighted in green
  • B0015 is highlighted in dark blue

Bpht_sequence.png

IDT report shows that the sequence is successfully synthesized:

Bpht_idt_2.png

Bpht_idt_1.png