Difference between revisions of "Part:BBa K1898150"

 
(→‎Synthesis Report)
 
(7 intermediate revisions by the same user not shown)
Line 3: Line 3:
 
<partinfo>BBa_K1898150 short</partinfo>
 
<partinfo>BBa_K1898150 short</partinfo>
  
−
BBa_K880005 consists of strong promoter and strong rbs to maximize protein production. This construct codes for ch25h, a catalyst for the conversion of cholesterol to 25 hydroxycholesterol, and 10x histidine tag for protein purification. BBa__B0015 is a double terminator used to stop transcription.  
+
BBa_K880005 consists of a strong promoter and strong RBS and was used to maximize protein production. This construct codes for CH25H (cholesterol 25-Hydroxylase) and a 10x Histidine-tag. CH25H is a catalyst that will make 25 hydroxycholesterol from cholesterol and 10x Histidine-tag is used for protein purification. BBa__B0015 is a double terminator used to stop transcription.  
−
 
+
−
<!-- Add more about the biology of this part here
+
−
===Usage and Biology===
+
  
 
<!-- -->
 
<!-- -->
Line 17: Line 14:
 
<partinfo>BBa_K1898150 parameters</partinfo>
 
<partinfo>BBa_K1898150 parameters</partinfo>
 
<!-- -->
 
<!-- -->
 +
 +
 +
===Synthesis Report===
 +
This construct is synthesized by IDT. The following sequence was sent to IDT to synthesize, and each feature is annotated as shown below:
 +
*Prefix and suffix are highlighted in orange
 +
*BBa_K880005 is highlighted in red
 +
*CH5H is highlighted in light blue
 +
*10x Histidine is highlighted in green
 +
*B0015 is highlighted in dark blue
 +
 +
https://static.igem.org/mediawiki/parts/8/8d/Bcht_idt.png
 +
 +
 +
IDT report shows that the sequence is successfully synthesized:
 +
 +
https://static.igem.org/mediawiki/parts/a/a4/Idt_bcht_1.png
 +
 +
https://static.igem.org/mediawiki/parts/9/96/Idt_bcht_2.png

Latest revision as of 15:27, 18 October 2016


Strong promoter + Strong RBS + CH25H + 10x His tag + Double terminator

BBa_K880005 consists of a strong promoter and strong RBS and was used to maximize protein production. This construct codes for CH25H (cholesterol 25-Hydroxylase) and a 10x Histidine-tag. CH25H is a catalyst that will make 25 hydroxycholesterol from cholesterol and 10x Histidine-tag is used for protein purification. BBa__B0015 is a double terminator used to stop transcription.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]



Synthesis Report

This construct is synthesized by IDT. The following sequence was sent to IDT to synthesize, and each feature is annotated as shown below:

  • Prefix and suffix are highlighted in orange
  • BBa_K880005 is highlighted in red
  • CH5H is highlighted in light blue
  • 10x Histidine is highlighted in green
  • B0015 is highlighted in dark blue

Bcht_idt.png


IDT report shows that the sequence is successfully synthesized:

Idt_bcht_1.png

Idt_bcht_2.png