Difference between revisions of "Part:BBa K1897023"
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<partinfo>BBa_K1897023 short</partinfo> | <partinfo>BBa_K1897023 short</partinfo> | ||
− | The construct contains | + | The construct contains a shorter version of the promoter region for wild-type lldPRD operon ([[Part:BBa_K1897037]]) derived from ([[Part:BBa_K822000]]) with a medium RBS flanked by non-coding sequences ([[Part:BBa_K1897032]]), followed by a coding sequence of ALR ([[Part:BBa_K1172901]]) and a lambda t0 terminator ([[Part:BBa_K1897030]]). |
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Revision as of 13:37, 18 October 2016
p62-32-ALR-Terminator
The construct contains a shorter version of the promoter region for wild-type lldPRD operon (Part:BBa_K1897037) derived from (Part:BBa_K822000) with a medium RBS flanked by non-coding sequences (Part:BBa_K1897032), followed by a coding sequence of ALR (Part:BBa_K1172901) and a lambda t0 terminator (Part:BBa_K1897030).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 501
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 425
Illegal BamHI site found at 1127 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 543
Illegal AgeI site found at 843 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 300