Difference between revisions of "Part:BBa K1993034"
LittleCloud (Talk | contribs) |
|||
| Line 3: | Line 3: | ||
<partinfo>BBa_K1993034 short</partinfo> | <partinfo>BBa_K1993034 short</partinfo> | ||
| − | In order to construct our device easily | + | In order to construct our device easily by Gateway technology, we constructed a composite part expressing IRES and eGFP which is convenient for running Gateway BP and LR reactions. |
We introduced Internal ribosome entry site (IRES) (Details can be seen from [https://parts.igem.org/Part:BBa_K1993016 BBa_K1993016]) to ensure the translation initiation in an end-independent manner, as part of the greater process of protein synthesis. | We introduced Internal ribosome entry site (IRES) (Details can be seen from [https://parts.igem.org/Part:BBa_K1993016 BBa_K1993016]) to ensure the translation initiation in an end-independent manner, as part of the greater process of protein synthesis. | ||
We also added the gene of eGFP(Details can be seen from [https://parts.igem.org/Part:BBa_K1993017 BBa_K1993017]) following IRES, which is a modified form of GFP frequently used as a reporter of expression. By constructing such a composite part, we could run our Gateway BP and LR reactions more easily through adding the Gateway loci to both end of that composite part. | We also added the gene of eGFP(Details can be seen from [https://parts.igem.org/Part:BBa_K1993017 BBa_K1993017]) following IRES, which is a modified form of GFP frequently used as a reporter of expression. By constructing such a composite part, we could run our Gateway BP and LR reactions more easily through adding the Gateway loci to both end of that composite part. | ||
Latest revision as of 13:12, 18 October 2016
IRES-eGFP
In order to construct our device easily by Gateway technology, we constructed a composite part expressing IRES and eGFP which is convenient for running Gateway BP and LR reactions. We introduced Internal ribosome entry site (IRES) (Details can be seen from BBa_K1993016) to ensure the translation initiation in an end-independent manner, as part of the greater process of protein synthesis. We also added the gene of eGFP(Details can be seen from BBa_K1993017) following IRES, which is a modified form of GFP frequently used as a reporter of expression. By constructing such a composite part, we could run our Gateway BP and LR reactions more easily through adding the Gateway loci to both end of that composite part.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]