Difference between revisions of "Part:BBa K1993034"

 
 
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<partinfo>BBa_K1993034 short</partinfo>
 
<partinfo>BBa_K1993034 short</partinfo>
  
IRES-eGFP
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In order to construct our device easily by Gateway technology, we constructed a composite part expressing IRES and eGFP which is convenient for running Gateway BP and LR reactions.
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We introduced Internal ribosome entry site (IRES) (Details can be seen from [https://parts.igem.org/Part:BBa_K1993016 BBa_K1993016]) to ensure the translation initiation in an end-independent manner, as part of the greater process of protein synthesis.
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We also added the gene of eGFP(Details can be seen from [https://parts.igem.org/Part:BBa_K1993017 BBa_K1993017]) following IRES, which is a modified form of GFP frequently used as a reporter of expression. By constructing such a composite part, we could run our Gateway BP and LR reactions more easily through adding the Gateway loci to both end of that composite part.
  
 
<!-- Add more about the biology of this part here
 
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Latest revision as of 13:12, 18 October 2016


IRES-eGFP

In order to construct our device easily by Gateway technology, we constructed a composite part expressing IRES and eGFP which is convenient for running Gateway BP and LR reactions. We introduced Internal ribosome entry site (IRES) (Details can be seen from BBa_K1993016) to ensure the translation initiation in an end-independent manner, as part of the greater process of protein synthesis. We also added the gene of eGFP(Details can be seen from BBa_K1993017) following IRES, which is a modified form of GFP frequently used as a reporter of expression. By constructing such a composite part, we could run our Gateway BP and LR reactions more easily through adding the Gateway loci to both end of that composite part.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]