Difference between revisions of "Part:BBa K1898150"

Revision as of 12:15, 18 October 2016

Strong promoter + Strong RBS + CH25H + 10x His tag + Double terminator

BBa_K880005 consists of a strong promoter and strong RBS and was used to maximize protein production. This construct codes for CH25H (cholesterol 25-Hydroxylase) and a 10x Histidine-tag. CH25H is a catalyst that will make 25 hydroxycholesterol from cholesterol and 10x Histidine-tag is used for protein purification. BBa__B0015 is a double terminator used to stop transcription.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Synthesis Report

This construct is synthesized by IDT. The following sequence was sent to IDT to synthesize, and each feature is annotated as shown below:

Prefix and suffix are highlighted in orange.

IDT report shows that the sequence is successfully synthesized:

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K1898150 short</partinfo>
-Strong promoter and strong rbs (BBa_K880005) are used to maximize protein production. This construct codes for CH25H (cholesterol 25-Hydroxylase) and a 10x Histidine Tag. CH25H is a catalyst that will make 25 hydroxycholesterol from cholesterol and 10x Histidine tag is used for protein purification. BBa__B0015 is a double terminator used to stop transcription.
+BBa_K880005 consists of a strong promoter and strong RBS and was used to maximize protein production. This construct codes for CH25H (cholesterol 25-Hydroxylase) and a 10x Histidine-tag. CH25H is a catalyst that will make 25 hydroxycholesterol from cholesterol and 10x Histidine-tag is used for protein purification. BBa__B0015 is a double terminator used to stop transcription.
 <!-- -->