Difference between revisions of "Part:BBa K1898100:Design"
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| − | CH25H was synthesized by IDT | + | CH25H was synthesized by IDT and the primers were synthesized by Tri-I Biotech. |
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Latest revision as of 11:48, 18 October 2016
ch25h, cholesterol 25 hydroxylase
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
CH25H was ordered with one base pair changed to remove an internal cutting site and the stop codon was removed as well.
PCR primers were designed to clone CH25H into a Biobrick backbone
forward: 5' ATATgAATTCgCggCCg 3' (17)
reverse: 5' ATATCTgCAgCggCC 3' (15)
Source
CH25H was synthesized by IDT and the primers were synthesized by Tri-I Biotech.