Difference between revisions of "Part:BBa K1998001"

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===Overview===
 
===Overview===
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This composite part is made up of YCF54 and ChlM.  
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This composite part is made up of the genes YCF54 and ChlM. They come from the chlorophyll biosynthetic pathway of Chlamydomonas reinhardtii. The two genes Mg-protoporphyrin IX monomethyl ester (oxidative) cyclase (YCF54) and Magnesium-protoporphyrin O-methyltransferase (ChlM). When combined in Operon 3 of our chlorophyll biosynthetic pathway they are involved in converting protoporphyrin IX to protoin the presence of NADPH and O2.
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ChlM - Mg protoporphyrin IX S-adenosyl methionine O-methyl transferase
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<html><center><img src="https://static.igem.org/mediawiki/2016/0/0f/T--Macquarie_Australia--ChlorophyllBiosynthesisDiagram.png" alt="ChlorophyllBiosynthesisDiagram" height="50%"width="75%"></center></html>
 
<html><center><img src="https://static.igem.org/mediawiki/2016/0/0f/T--Macquarie_Australia--ChlorophyllBiosynthesisDiagram.png" alt="ChlorophyllBiosynthesisDiagram" height="50%"width="75%"></center></html>
  

Revision as of 11:36, 18 October 2016


YCF54 - ChlM

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 912
    Illegal BamHI site found at 316
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 478


Overview

This composite part is made up of the genes YCF54 and ChlM. They come from the chlorophyll biosynthetic pathway of Chlamydomonas reinhardtii. The two genes Mg-protoporphyrin IX monomethyl ester (oxidative) cyclase (YCF54) and Magnesium-protoporphyrin O-methyltransferase (ChlM). When combined in Operon 3 of our chlorophyll biosynthetic pathway they are involved in converting protoporphyrin IX to protoin the presence of NADPH and O2.

ChlorophyllBiosynthesisDiagram

Biology & Literature

Protein information

YCF54
mass: 17.08kDa
sequence: MAPAAASADKATAAEYYALVCNAEWFFMDPQNESVAEQLREKVRFFKEQN KERDFFIVPNPKWLDAKFPEQAKQVKRPCVALVSTDKMWITFMKLRLDRV LKIDLKSMPASEVLAAGEALPDFKPDGKWTAPYARYTPGWWNVFLPNH


ChlM
mass: 30.45kDa sequence: MASEIAQTADVGSLTFAVGGVGAVVGLGALLVATDHQKRRSEQMKSFDGD EKEAVKDYFNTAGFERWRKIYGETDEVNKVQLDIRTGHAQTVDKVLRWVD EEGSVQGITVADCGCGTGSLAIQLALRGAAVSASDISAAMASEAEQRYQQ AVAAGQGKAPKVAPKFEALDLESVKGKYDTVTCLDVMIHYPQDKVDAMIT HLAGLSDRRLIISFAPKTLSYSILKRIGELFPGPSKATRAYLHREEDVEAALK RAGFKVTKREMTATSFYFSRLLEAIRE

References