Difference between revisions of "Part:BBa K2144008"
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This Biobrick also contains GB1 acting as a solubility tag  and a His-tag to enable IMAC-purification.
 
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===Usage and Biology===
 
===Usage and Biology===
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This Biobrick is a truncated version of the enzyme where  the transmembrane domain (amino acids 1-59) is not included in the coding sequence. The BioBrick has also been fused with the Protein G b1 Domain (GB1) acting as a solubility tag  and a His-tag to enable IMAC-purification.
  
 
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<partinfo>BBa_K2144008 parameters</partinfo>
 
<partinfo>BBa_K2144008 parameters</partinfo>
 
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===References===
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[1] Popp, M. W.-L. and Ploegh, H. L. (2011), Making and Breaking Peptide Bonds: Protein Engineering Using Sortase. Angew. Chem. Int. Ed., 50: 5024–503
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[2] Westerlund, K. Karlstrom, A., Honarvar, H., Tolmachev, V. Design, Preparation, and Characterization of PNA-Based Hybridization Probes for Affibody-Molecule-Mediated Pretargeting. Bioconjugate Chem., 2015, 26 (8), pp 1724–1736

Revision as of 11:17, 18 October 2016


Coding sequence for Sortase A with His-tag

Sortase A is a bacterial enzyme with the ability to break and form new peptide bonds. The key feature of the enzyme is the specific conjugation reaction it carries out, where the enzyme recognizes a specific amino acid sequence, a so called sorting motif (LPXTG motif in the case of S.aureus) and conjugate this sequence with another unit carrying an oligo glycine motif. A new peptide bond is formed.


Usage and Biology

This Biobrick is a truncated version of the enzyme where the transmembrane domain (amino acids 1-59) is not included in the coding sequence. The BioBrick has also been fused with the Protein G b1 Domain (GB1) acting as a solubility tag and a His-tag to enable IMAC-purification.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

[1] Popp, M. W.-L. and Ploegh, H. L. (2011), Making and Breaking Peptide Bonds: Protein Engineering Using Sortase. Angew. Chem. Int. Ed., 50: 5024–503

[2] Westerlund, K. Karlstrom, A., Honarvar, H., Tolmachev, V. Design, Preparation, and Characterization of PNA-Based Hybridization Probes for Affibody-Molecule-Mediated Pretargeting. Bioconjugate Chem., 2015, 26 (8), pp 1724–1736