Difference between revisions of "Part:BBa K1898150:Design"
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===Design Notes=== | ===Design Notes=== | ||
We performed one silent point mutation on the CH25H DNA to remove one internal cutting site. The stop codon in CH25H was removed as well. | We performed one silent point mutation on the CH25H DNA to remove one internal cutting site. The stop codon in CH25H was removed as well. | ||
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Primers were synthesized by Tri-I biotech. | Primers were synthesized by Tri-I biotech. | ||
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===References=== | ===References=== | ||
Revision as of 10:56, 18 October 2016
Strong promoter + Strong RBS + CH25H + 10x His tag + Double terminator
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We performed one silent point mutation on the CH25H DNA to remove one internal cutting site. The stop codon in CH25H was removed as well.
Source
this DNA is synthesized by IDT.
The following primers are designed and used to move the DNA synthesized by IDT into iGEM Biobrick: forward: 5' ATATgAATTCgCggCCg 3' (17) reverse: 5' ATATCTgCAgCggCC 3' (15)
Primers were synthesized by Tri-I biotech.