Difference between revisions of "Part:BBa K1898150:Design"
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===Design Notes===
 
===Design Notes===
−
We made sure that there was no Ecor1, Xbal, Spel1, and Pst1 cutting sites in the DNA.  
+
We performed one silent point mutation on the CH25H DNA to remove one internal cutting site. The stop codon in CH25H was removed as well.  
  
  
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this DNA is synthesized by IDT.  
 
this DNA is synthesized by IDT.  
  
−
The following primers are designed and used to move the DNA from IDT to iGEM Biobrick:
+
The following primers are designed and used to move the DNA synthesized by IDT into iGEM Biobrick:
 +
forward: 5' ATATgAATTCgCggCCg 3' (17)
 +
reverse: 5' ATATCTgCAgCggCC 3' (15)
  
 +
Primers were synthesized by Tri-I biotech.
  
 
===References===
 
===References===

Revision as of 10:55, 18 October 2016


Strong promoter + Strong RBS + CH25H + 10x His tag + Double terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Design Notes

We performed one silent point mutation on the CH25H DNA to remove one internal cutting site. The stop codon in CH25H was removed as well.


Source

this DNA is synthesized by IDT.

The following primers are designed and used to move the DNA synthesized by IDT into iGEM Biobrick: forward: 5' ATATgAATTCgCggCCg 3' (17) reverse: 5' ATATCTgCAgCggCC 3' (15)

Primers were synthesized by Tri-I biotech.

References