Difference between revisions of "Part:BBa K1898550"

Revision as of 10:31, 18 October 2016

Strong promoter + Strong RBS + GFP + 10x Histidine tag + double terminator

Strong promoter and strong rbs (BBa_K880005) are used to maximize protein output. The ORF includes green fluorescence protein and a 10x Histidine tag for protein purification. BBa_B0015 consists of a double terminator to end transcription.

This part can be used to test protein purification method or be used as a way to quantify protein expression level.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 705

synthesis report

This construct is synthesized by IDT. The following sequence was sent to IDT to synthesize, and each feature is annotated as shown below:

IDT report shows that the sequence is successfully synthesized:

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K1898550 short</partinfo>
-Strong promoter and strong rbs (BBa_K880005) is used to maximize protein output. The ORF includes green fluorescence protein and a 10x Histidine tag for protein purification. BBa_B0015 consists of a double terminator to end transcription.
+Strong promoter and strong rbs (BBa_K880005) are used to maximize protein output. The ORF includes green fluorescence protein and a 10x Histidine tag for protein purification. BBa_B0015 consists of a double terminator to end transcription.
 This part can be used to test protein purification method or be used as a way to quantify protein expression level.