Difference between revisions of "Part:BBa K1890031"

 
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<partinfo>BBa_K1890031 short</partinfo>
 
<partinfo>BBa_K1890031 short</partinfo>
  
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Enter a long description of the part so that users of your part know what it is, what it does, and how to use it in their projects.
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<h2>Introduction</h2>
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BolA is a gene that controls the morphology of E. coli in the stress response (Santos, Freire, Vicente, & Arraiano, 1999). By overexpressing this gene at 37°C, naturally rod-shaped E. coli cells will become spherical (Aldea, Hernandez-Chico, De La Campa, Kushner, & Vicente, 1988). In this part, the gene is under control of a constitutive promoter, RBS and terminators.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 09:34, 18 October 2016


BolA gene with strong constitutive promoter, RBS and terminator

Introduction

BolA is a gene that controls the morphology of E. coli in the stress response (Santos, Freire, Vicente, & Arraiano, 1999). By overexpressing this gene at 37°C, naturally rod-shaped E. coli cells will become spherical (Aldea, Hernandez-Chico, De La Campa, Kushner, & Vicente, 1988). In this part, the gene is under control of a constitutive promoter, RBS and terminators.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 158
  • 1000
    COMPATIBLE WITH RFC[1000]