Difference between revisions of "Part:BBa K1993034"

Revision as of 09:23, 18 October 2016

IRES-eGFP

In order to construct our device easily since we constructed our plasmids by Gateway technology, we constructed a composite part expressing IRES and eGFP which is convenient for running Gateway BP and LR reactions. We introduced Internal ribosome entry site (IRES) (Details can be seen from BBa_K1993016) to ensure the translation initiation in an end-independent manner, as part of the greater process of protein synthesis. We also added the gene of eGFP(Details can be seen from BBa_K1993017) following IRES, which is a modified form of GFP frequently used as a reporter of expression. By constructing such a composite part, we could run our Gateway BP and LR reactions more easily through adding the Gateway loci to both end of that composite part.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K1993034 short</partinfo>
-IRES-eGFP
+In order to construct our device easily since we constructed our plasmids by Gateway technology, we constructed a composite part expressing IRES and eGFP which is convenient for running Gateway BP and LR reactions.
+We introduced Internal ribosome entry site (IRES) (Details can be seen from [https://parts.igem.org/Part:BBa_K1993016 BBa_K1993016]) to ensure the translation initiation in an end-independent manner, as part of the greater process of protein synthesis.
+We also added the gene of eGFP(Details can be seen from [https://parts.igem.org/Part:BBa_K1993017 BBa_K1993017]) following IRES, which is a modified form of GFP frequently used as a reporter of expression. By constructing such a composite part, we could run our Gateway BP and LR reactions more easily through adding the Gateway loci to both end of that composite part.
 <!-- Add more about the biology of this part here