Difference between revisions of "Part:BBa K2074032"

Revision as of 08:29, 18 October 2016

Cry11Aa(Codon optimization)+Extended FMDV 2A+Cyt2(Codon optimization)

Cry10Aa and Cyt2 are protein from Bacillus thuringiensis (Bt),which have toxicity to the larave of mosquito.We used 2A system to express two protein at the same time in Chlamydomonas reinhardti. These two proteins express at the same time can enhance the toxicity to the larvae of mosquito.

Fig.1 Cry10Aa and Cyt2 Toxins Group

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 2195
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 517
Illegal NgoMIV site found at 1681
Illegal NgoMIV site found at 2029
Illegal NgoMIV site found at 2329
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 49
Illegal BsaI.rc site found at 244
Illegal BsaI.rc site found at 1459
Illegal SapI.rc site found at 1440

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K2074032 short</partinfo>
-cry10Aa and cyt2 are protein from Bacillus thuringiensis (or Bt),which have toxicity to the larve of mosquito.we use 2A system to express two protein at the same time in Chlamydomonas reinhardti.These two protein express at the same time can enhance the  toxicity to the larve of mosquito.
+Cry10Aa and Cyt2 are protein from Bacillus thuringiensis (Bt),which have toxicity to the larave of mosquito.We used 2A system to express two protein at the same time in Chlamydomonas reinhardti. These two proteins express at the same time can enhance the  toxicity to the larvae of mosquito.
+[[File:2016FAFUCHINADevice5.png|800px|thumb|center|'''Fig.1''' Cry10Aa and Cyt2 Toxins Group]]
 <!-- Add more about the biology of this part here
 ===Usage and Biology===