Difference between revisions of "Part:BBa K2074015"
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<partinfo>BBa_K2074015 short</partinfo> | <partinfo>BBa_K2074015 short</partinfo> | ||
| − | 2A peptides system was firstly discovered in Foot-and-mouth disease virus (FMDV). 2A peptide is a linker which used to separate functional proteins between upstream and downstream, and the length is about 18-22 amino acids long. ‘Ribosomal skip’ occurs during translation that result in production of independent peptides or proteins from one polycistronic. After cleavage, the 2A peptide remains fused to the C-terminus of the upstream protein when the proline is linked to the N-terminus of the downstream as | + | 2A peptides system was firstly discovered in Foot-and-mouth disease virus (FMDV). 2A peptide is a linker which used to separate functional proteins between upstream and downstream, and the length is about 18-22 amino acids long. ‘Ribosomal skip’ occurs during translation that result in production of independent peptides or proteins from one polycistronic. After cleavage, the 2A peptide remains fused to the C-terminus of the upstream protein when the proline is linked to the N-terminus of the downstream as Figure. According to the Thomas’s research, 2A peptide system has proved the high-efficiency of co-expression and cleavage in Chlamydomonas |
[[File:2016FAFUCHINADevice3.png|800px|thumb|center|'''Fig.1''' The Mechanism of 2A Peptide System]] | [[File:2016FAFUCHINADevice3.png|800px|thumb|center|'''Fig.1''' The Mechanism of 2A Peptide System]] | ||
Latest revision as of 08:03, 18 October 2016
TaV 2A for Chlamydomonas reinhardtii
2A peptides system was firstly discovered in Foot-and-mouth disease virus (FMDV). 2A peptide is a linker which used to separate functional proteins between upstream and downstream, and the length is about 18-22 amino acids long. ‘Ribosomal skip’ occurs during translation that result in production of independent peptides or proteins from one polycistronic. After cleavage, the 2A peptide remains fused to the C-terminus of the upstream protein when the proline is linked to the N-terminus of the downstream as Figure. According to the Thomas’s research, 2A peptide system has proved the high-efficiency of co-expression and cleavage in Chlamydomonas
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]