Difference between revisions of "Part:BBa K2097000"
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<partinfo>BBa_K2097000 short</partinfo> | <partinfo>BBa_K2097000 short</partinfo> | ||
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| − | CpxA-CpxR is a two-component mechanism that is activated at pH 7.4 and repressed at pH 6.0. CpxA is an intermembrane protein that autophosphorylates at a certain external pH, CpxR (a kinase) then gets phosphorylated by CpxA and acts as a transcription factor. This system originally is a transcription factor for the virF gene, but virF was replaced with a reporter. The original sequence was found in <i>Shigella sonnei</i>, but <i>E. coli</i> has a homolog of these proteins so all that is required on the construct is the appropriate prefix/suffix and CpxR binding site. Used <a href="https://parts.igem.org/Part:BBa_K2097002">BBa_K2097002</a> as the control series. | + | CpxA-CpxR is a two-component mechanism that is activated at pH 7.4 and repressed at pH 6.0. CpxA is an intermembrane protein that autophosphorylates at a certain external pH, CpxR (a kinase) then gets phosphorylated by CpxA and acts as a transcription factor. This system originally is a transcription factor for the virF gene, but virF was replaced with a reporter. The original sequence was found in <i>Shigella sonnei</i>, but <i>E. coli</i> has a homolog of these proteins so all that is required on the construct is the appropriate prefix/suffix and CpxR binding site. Used <a href="https://parts.igem.org/Part:BBa_K2097002">BBa_K2097002</a> as the control series.</html> |
<h3>Usage and Biology</h3><p></p> | <h3>Usage and Biology</h3><p></p> | ||
Revision as of 07:19, 18 October 2016
CpxR binding site attached to a yellow-green color protein (YGCP) acts as a neutral pH indicator.
CpxA-CpxR is a two-component mechanism that is activated at pH 7.4 and repressed at pH 6.0. CpxA is an intermembrane protein that autophosphorylates at a certain external pH, CpxR (a kinase) then gets phosphorylated by CpxA and acts as a transcription factor. This system originally is a transcription factor for the virF gene, but virF was replaced with a reporter. The original sequence was found in Shigella sonnei, but E. coli has a homolog of these proteins so all that is required on the construct is the appropriate prefix/suffix and CpxR binding site. Used BBa_K2097002 as the control series.
Usage and Biology
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]