Difference between revisions of "Part:BBa K2100037:Design"
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===Source=== | ===Source=== | ||
− | Comes from some genomic sequence. | + | Comes from some mammalian genomic sequence. |
===References=== | ===References=== |
Latest revision as of 23:20, 17 October 2016
pEXPR TRE - TALER14
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 6
Illegal EcoRI site found at 322
Illegal EcoRI site found at 344
Illegal EcoRI site found at 518
Illegal XbaI site found at 30
Illegal XbaI site found at 495 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 6
Illegal EcoRI site found at 322
Illegal EcoRI site found at 344
Illegal EcoRI site found at 518 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 6
Illegal EcoRI site found at 322
Illegal EcoRI site found at 344
Illegal EcoRI site found at 518
Illegal BamHI site found at 641
Illegal XhoI site found at 584 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 6
Illegal EcoRI site found at 322
Illegal EcoRI site found at 344
Illegal EcoRI site found at 518
Illegal XbaI site found at 30
Illegal XbaI site found at 495 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 6
Illegal EcoRI site found at 322
Illegal EcoRI site found at 344
Illegal EcoRI site found at 518
Illegal XbaI site found at 30
Illegal XbaI site found at 495 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 656
Design Notes
This composite part expression vector was created by an LR reaction. It is a promoter (flanked by L4, R1 sites) and a gene (flanked by L1, L2 sites) cloned into a backbone that has a negative selection marker between R4 and R2 sites. This part adheres to RFC 65 for recombination based cloning of mammalian parts.
Source
Comes from some mammalian genomic sequence.