Difference between revisions of "Part:BBa K1993032"

Latest revision as of 19:37, 17 October 2016

CXCR4-T2A-Luciferase

With the purpose of increasing homing efficiency of MSCs and illustrating their distribution, we had constructed a composite part that containing coding sequences of chemokine receptor CXCR4 and bioluminescence Luciferase. It’s also convenient for us to construct our plasmids through Gateway technology.

CXCR4 is responsible for directing T lymphocytes to the lesions whose ligand is CXCL12, one of the most common chemokines in various inflammatory diseases, especially in inflammatory bowel disease(IBD). (Details could be seen on BBa_K1993013).

Luciferase is a kind of oxidative enzymes that produce bioluminescence which enable us to observe cells in vivo. (Details could be seen on BBa_K1993015).

T2A is a self-cleaving 2A peptide (The average length is 18–22 amino acids) that could be applied in expression of more than one gene in cells. (Details could be seen on BBa_K1993019)

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 1350
Illegal BamHI site found at 494
Illegal BamHI site found at 1893
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 5: / Line 5: @@
 With the purpose of increasing homing efficiency of MSCs and illustrating their distribution, we had constructed a composite part that containing coding sequences of chemokine receptor CXCR4 and bioluminescence Luciferase. It’s also convenient for us to construct our plasmids through Gateway technology.
-CXCL12 is one of the most common chemokines in various inflammatory diseases, especially in inflammatory bowel disease(IBD). CXCR4, its corresponding chemokine receptor, is responsible for directing T lymphocytes to the lesions. (Details could be seen on [https://parts.igem.org/Part:BBa_K1993013 BBa_K1993013]).
+CXCR4 is responsible for directing T lymphocytes to the lesions whose ligand is CXCL12, one of the most common chemokines in various inflammatory diseases, especially in inflammatory bowel disease(IBD). (Details could be seen on [https://parts.igem.org/Part:BBa_K1993013 BBa_K1993013]).
-Luciferase is a kind of oxidative enzymes that produce bioluminescence which enable us observing cells in vivo. (Details could be seen on [https://parts.igem.org/Part:BBa_K1993015 BBa_K1993015]).
+Luciferase is a kind of oxidative enzymes that produce bioluminescence which enable us to observe cells in vivo. (Details could be seen on [https://parts.igem.org/Part:BBa_K1993015 BBa_K1993015]).
 T2A is a self-cleaving 2A peptide (The average length is 18–22 amino acids) that could be applied in expression of more than one gene in cells. (Details could be seen on [https://parts.igem.org/Part:BBa_K1993019 BBa_K1993019])