Difference between revisions of "Part:BBa K1937002"

Revision as of 18:58, 17 October 2016

OriKan: a composite part to turn any pSB1C3 into a Bacillus plasmid

Part: BBa_K1937002 (pSB1C3-OriKan)
(Chassis E. coli/B. subtilis, carrier plasmid pSB1C3)
Length: 2510 bp

While Bacillus subtilis is of huge interest for a growing number of iGEM projects, it is not easy to develop new parts as it is required that they are registered after sub-cloning in the E. coli plasmid pSB1C3. During the iGEM Toulouse 2016 project, we had the idea to create a part that could turn any pSB1C3-based plasmid in a shuttle vector (E. coli/B. subtilis). This BioBrick is a part developed by the Toulouse 2016 iGEM team (http://2016.igem.org/Team:Toulouse_France).

More information available at http://2016.igem.org/Team:Toulouse_France/Description

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 2196
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 1807
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 522
Illegal SapI.rc site found at 2020

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K1937002 short</partinfo>
-<b>Part: BBa_K1937002 (pSB1C3-OriKan)<br>
+<b>Part: BBa_K1937002 (pSB1C3-OriKan)<br></b>
 (Chassis <i>E. coli/B. subtilis</i>, carrier plasmid pSB1C3)<br>
-Length: 2510 bp</b>
+Length: 2510 bp<br><br>
 While <i>Bacillus subtilis</i> is of huge interest for a growing number of iGEM projects, it is not easy to develop new parts as it is required that they are registered after sub-cloning in the <i>E. coli</i> plasmid pSB1C3. During the iGEM Toulouse 2016 project, we had the idea to create a part that could turn any pSB1C3-based plasmid in a shuttle vector (<i>E. coli/B. subtilis</i>).