Difference between revisions of "Part:BBa K2097001"
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[[File:T--Austin_UTexas--Cpx_pH_Culture_Tubes_2.png|thumb|left|600px| Figure 1. Testing the CpxR Construct in pH 6-9. From left to right is Control pH 6-9 and then Experimental pH 6-9. These are showing the gradient change in expression accordingly with the change of pH due to a pH-dependent promotor compared to consistent expression accordingly with a promoter that is always "on".]]
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[[File:T--Austin_UTexas--BCPplate.png|thumb|left|600px| Figure 3. Control amilCP. This control was used to compare the color intensity to the pH sensitive P-atp2 while testing with a range of pH values. This part was documented to not be affected by changes in pH.]]
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Revision as of 18:31, 17 October 2016


Blue chromoprotein with strong promoter and RBS

This is a composite part made up of BBa_K592009 (blue chromoprotein) and BBa_K608002 (a strong RBS and promoter). The protein exhibits a strong blue/purple color when expressed. BBa_K608002 is made up of a promoter, BBa_J23104, and an RBS, BBa_B003.


Usage and Biology

BBa_K592009 was created by Uppsala-Sweden 2011. It comes from the coral Acropora millepora, amilCP. BBa_K608002 was created by Freiburg 2011.

Figure 3. Control amilCP. This control was used to compare the color intensity to the pH sensitive P-atp2 while testing with a range of pH values. This part was documented to not be affected by changes in pH.

<p> Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]