Difference between revisions of "Part:BBa K1968001"

Latest revision as of 18:26, 17 October 2016

Synechocystis Pcpc560 Phytobrick promoter

Pcpc560 stands for the 560bp region found upstream of the cpcB gene encoding the beta subunit of c-phycocyanin in Synechocystis sp. strain PCC 6803. This region has been reported to act as a promoter that is able of producing heterologous proteins at a level up to 15% of total soluble protein in Synechocystis, which is comparable to levels achieved in E. coli (1). In light of this, this genomic region has been termed as the Pcpc560 “super-strong” promoter. Pcpc560 contains two predicted promoters and 14 predicted transcription factor binding sites (1). A native RBS is present in the Pcpc560 sequence, meaning that this part can be cloned directly in front of a reporter (1).

(1) Zhou, J., Zhang, H., Meng, H., Zhu, Y., Bao, G., Zhang, Y., . . . Ma, Y. (2014). Discovery of a super-strong promoter enables efficient production of heterologous proteins in cyanobacteria. Sci Rep, 4, 4500. doi: 10.1038/srep04500

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 2

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K1968001 short</partinfo>
-Pcpc560 stands for the  560bp region found upstream of the cpcB gene encoding the beta subunit of c-phycocyanin in Synechocystis sp. strain PCC 6803. This region has been reported to act as a promoter that is able of producing heterologous proteins at a level up to 15% of total soluble protein in Synechocystis, which is comparable to levels achieved in E. coli (Zhou et al., 2014). In light of this, this genomic region has been termed as the Pcpc560 “super-strong” promoter.
+Pcpc560 stands for the  560bp region found upstream of the cpcB gene encoding the beta subunit of c-phycocyanin in Synechocystis sp. strain PCC 6803. This region has been reported to act as a promoter that is able of producing heterologous proteins at a level up to 15% of total soluble protein in Synechocystis, which is comparable to levels achieved in E. coli (1). In light of this, this genomic region has been termed as the Pcpc560 “super-strong” promoter. Pcpc560 contains two predicted promoters and 14 predicted transcription factor binding sites (1). A native RBS is present in the Pcpc560 sequence, meaning that this part can be cloned directly in front of a reporter (1).
+(1) Zhou, J., Zhang, H., Meng, H., Zhu, Y., Bao, G., Zhang, Y., . . . Ma, Y. (2014). Discovery of a super-strong promoter enables efficient production of heterologous proteins in cyanobacteria. Sci Rep, 4, 4500. doi: 10.1038/srep04500