Difference between revisions of "Part:BBa K1937008"
Line 4: Line 4:
  
 
(Chassis <i>B. subtilis</i>, carrier plasmid pSBBS0K-mini)<br>
 
(Chassis <i>B. subtilis</i>, carrier plasmid pSBBS0K-mini)<br>
−
Length: 6558bp
+
Length: 6558bp<br>
−
<b>Background:</b>
+
<b>Background:</b><br>
 
This biobrick is an assembly of the K1937001 pSBBS0K-mini <i>B. subtilis</i> plasmid with the K1937007 part containing AF-A (a part with two genes encoding antifungal peptides: a cutted Metchnikowin and the D4E1 peptide under the control of the strong constitutive promoter pVeg).<br>
 
This biobrick is an assembly of the K1937001 pSBBS0K-mini <i>B. subtilis</i> plasmid with the K1937007 part containing AF-A (a part with two genes encoding antifungal peptides: a cutted Metchnikowin and the D4E1 peptide under the control of the strong constitutive promoter pVeg).<br>
  

Revision as of 17:43, 17 October 2016


AF-A antifungal operon in pSBBs0K-mini

(Chassis B. subtilis, carrier plasmid pSBBS0K-mini)
Length: 6558bp
Background:
This biobrick is an assembly of the K1937001 pSBBS0K-mini B. subtilis plasmid with the K1937007 part containing AF-A (a part with two genes encoding antifungal peptides: a cutted Metchnikowin and the D4E1 peptide under the control of the strong constitutive promoter pVeg).

This BioBrick is a part developed by the Toulouse 2016 iGEM team (http://2016.igem.org/Team:Toulouse_France)

BBa K1937007-map1.png

BBa K1937008-genemap.png Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 1228
    Illegal suffix found in sequence at 1256
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1228
    Illegal NheI site found at 250
    Illegal NheI site found at 1250
    Illegal SpeI site found at 1257
    Illegal PstI site found at 1271
    Illegal NotI site found at 1234
    Illegal NotI site found at 1264
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1228
    Illegal BglII site found at 970
    Illegal BglII site found at 5785
    Illegal BamHI site found at 1301
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 1228
    Illegal suffix found in sequence at 1257
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1228
    Illegal XbaI site found at 1243
    Illegal SpeI site found at 1257
    Illegal PstI site found at 1271
    Illegal NgoMIV site found at 2848
    Illegal AgeI site found at 5396
    Illegal AgeI site found at 6358
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 438
    Illegal BsaI.rc site found at 1026
    Illegal BsaI.rc site found at 2672
    Illegal BsaI.rc site found at 4111
    Illegal BsaI.rc site found at 6627
    Illegal SapI site found at 1589
    Illegal SapI.rc site found at 5609