Difference between revisions of "Part:BBa K1974021"
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<partinfo>BBa_K1974021 short</partinfo>
 
<partinfo>BBa_K1974021 short</partinfo>
  
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<h1>'''Introduction:'''</h1>
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[[File:|NCTU_H.png|800px|thumb|center|'''Figure 1.’’’T7 promoter+RBS+Hv1a+linker+His-tag+terminator ]]
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&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; By ligating the IPTG induced promoter T7 (BBa_ I712074), strong ribosome binding site (BBa_B0034), hv1a, linker, and the 6xHistag (BBa_ K1223006), we can express Hv1a, the toxin by IPTG induction
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&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; This year we create a revolutionary system that integrates biological pesticides, automatic detector, sprinkler, and IoT. We made a database that contains most of the spider toxins and selected the target toxins by programming. Omega-hexatoxin-Hv1a is coded for the venom of a spider, <i>Hadronyche versuta</i>.
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Revision as of 17:13, 17 October 2016

T7Promoter+RBS+Hv1a+linker+snowdrop-lectin+linker+6X His-Tag


Introduction:

[[File:|NCTU_H.png|800px|thumb|center|Figure 1.’’’T7 promoter+RBS+Hv1a+linker+His-tag+terminator ]]

       By ligating the IPTG induced promoter T7 (BBa_ I712074), strong ribosome binding site (BBa_B0034), hv1a, linker, and the 6xHistag (BBa_ K1223006), we can express Hv1a, the toxin by IPTG induction
       This year we create a revolutionary system that integrates biological pesticides, automatic detector, sprinkler, and IoT. We made a database that contains most of the spider toxins and selected the target toxins by programming. Omega-hexatoxin-Hv1a is coded for the venom of a spider, Hadronyche versuta.












Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]