Difference between revisions of "Part:BBa K1968015:Design"
(→Source) |
|||
Line 14: | Line 14: | ||
===Source=== | ===Source=== | ||
− | The sequence was provided by the Fungal Stock Genetic Center. It was PCR out from plasmid P444 from their collection. | + | The sequence was provided by the Fungal Stock Genetic Center. It was PCR out from plasmid P444 from their collection, it was then assembled into the PhytoBricks Universal Acceptor (BBa_P10500). The sequence of this part was confirmed by Sanger Sequencing |
− | + | ||
===References=== | ===References=== |
Latest revision as of 17:08, 17 October 2016
Phleomycin resistance cassette Phytobrick
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 379
Illegal NgoMIV site found at 440 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
The device has the A_E MoClo compatible phytobrick overhangs.
Source
The sequence was provided by the Fungal Stock Genetic Center. It was PCR out from plasmid P444 from their collection, it was then assembled into the PhytoBricks Universal Acceptor (BBa_P10500). The sequence of this part was confirmed by Sanger Sequencing