Difference between revisions of "Part:BBa K1993032"

Revision as of 16:42, 17 October 2016

CXCR4-T2A-Luciferase

With the purpose of increasing homing efficiency of MSCs and illustrating their distribution, we had constructed a composite part that containing coding sequences of chemokine receptor CXCR4 and bioluminescence Luciferase. It’s also convenient for us to construct our plasmids through Gateway technology.

CXCL12 is one of the most common chemokines in various inflammatory diseases, especially in inflammatory bowel disease(IBD). CXCR4, its corresponding chemokine receptor, is responsible for directing T lymphocytes to the lesions. (Details could be seen on BBa_K1993013).

Luciferase is a kind of oxidative enzymes that produce bioluminescence which enable us observing cells in vivo. (Details could be seen on BBa_K1993015).

T2A is a self-cleaving 2A peptide (The average length is 18–22 amino acids) that could be applied in expression of more than one gene in cells. (Details could be seen on BBa_K1993019)

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 1350
Illegal BamHI site found at 494
Illegal BamHI site found at 1893
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K1993032 short</partinfo>
-CXCR4-T2A-luciferase
+With the purpose of increasing homing efficiency of MSCs and illustrating their distribution, we had constructed a composite part that containing coding sequences of chemokine receptor CXCR4 and bioluminescence Luciferase. It’s also convenient for us to construct our plasmids through Gateway technology.
+CXCL12 is one of the most common chemokines in various inflammatory diseases, especially in inflammatory bowel disease(IBD). CXCR4, its corresponding chemokine receptor, is responsible for directing T lymphocytes to the lesions. (Details could be seen on [https://parts.igem.org/Part:BBa_K1993013 BBa_K1993013]).
+Luciferase is a kind of oxidative enzymes that produce bioluminescence which enable us observing cells in vivo. (Details could be seen on [https://parts.igem.org/Part:BBa_K1993015 BBa_K1993015]).
+T2A is a self-cleaving 2A peptide (The average length is 18–22 amino acids) that could be applied in expression of more than one gene in cells. (Details could be seen on [https://parts.igem.org/Part:BBa_K1993019 BBa_K1993019])
 <!-- Add more about the biology of this part here