Difference between revisions of "Part:BBa K1985015"
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<partinfo>BBa_K1985015 short</partinfo> | <partinfo>BBa_K1985015 short</partinfo> | ||
− | This part is an improved version of the Envirowire fusion protein( | + | This part is an improved version of the Envirowire fusion protein (BBa_K1739002), which was designed by the Kent 2015 iGEM team. The promoter BBa_J23104 has been removed.. It contains 2 segments, the CsgA signal sequence and Sup35NM. |
<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> |
Revision as of 15:23, 17 October 2016
pSB1A3-[AraC-pBAD]-[CsgA-SS-Sup35NM]
This part is an improved version of the Envirowire fusion protein (BBa_K1739002), which was designed by the Kent 2015 iGEM team. The promoter BBa_J23104 has been removed.. It contains 2 segments, the CsgA signal sequence and Sup35NM.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1205
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1144
- 23INCOMPATIBLE WITH RFC[23]Unknown
- 25INCOMPATIBLE WITH RFC[25]Unknown
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 961
Usage and Biology
For more information on biology and usage see part BBa_K1739002
Validation
The part was first validated with a diagnostic restriction digest using EcoRI and PstI and agarose gel electrophoresis. The expected band sizes from the digest were: xxxx for the plasmid backbone and xxx for the insert. A 1kB plus DNA marker was used to verify the sizes of the bands and it was confirmed that the correct plasmid had been produced.