Difference between revisions of "Part:BBa K2060000:Design"
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===Design Notes=== | ===Design Notes=== | ||
+ | One of the Cardiff_Wales iGEM instructors Daniel Pass, designed a [https://github.com/passdan/scriptdrop/blob/master/cas9_targeter.py bioinformatic tool] for the selection of appropriate guide RNAs that adhered to the following rules: | ||
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Used standard design considerations for guide RNAs | Used standard design considerations for guide RNAs | ||
Revision as of 14:44, 17 October 2016
CRISPR-Cas9 guide RNA targeting to 16S RNA
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 48
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
One of the Cardiff_Wales iGEM instructors Daniel Pass, designed a bioinformatic tool for the selection of appropriate guide RNAs that adhered to the following rules:
Used standard design considerations for guide RNAs
Source
E.coli 16S ribosomal RNA