Difference between revisions of "Part:BBa K2172012"

Latest revision as of 13:37, 17 October 2016

Tac Promoter-RBS-GST-Thrombin Protease-SmCPS1-TEV-GFP-Terminator

This part is a complete gene circuit that can be used to produce the enzyme SmCPS1. SmCPS1 is a key enzyme on the biosynthesis pathway of tanshinone (a compound used to treat cardiovascular diseases). The tac promoter, a strong hybrid promoter, is used to control and increase the expression levels of the target gene (SmCPS1), and is used in the expression of recombinant proteins, produced from the combination of promoters from the trp and lac operons. The GST label is used to purify the recombinant protein. The GFP is used to measure the expression level of the part.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 2654
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 1415
Illegal BamHI site found at 747
Illegal BamHI site found at 1013
Illegal XhoI site found at 1149
Illegal XhoI site found at 1173
Illegal XhoI site found at 2466
Illegal XhoI site found at 2676
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 818
Illegal NgoMIV site found at 2235
Illegal NgoMIV site found at 2799
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 159

Revision as of 03:44, 15 October 2016 (view source) Registry (Talk \| contribs)		Latest revision as of 13:37, 17 October 2016 (view source) Xiaobowen (Talk \| contribs)
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	<partinfo>BBa_K2172012 short</partinfo>		<partinfo>BBa_K2172012 short</partinfo>

−	This part is a gene circuit that can be used to produce the enzyme SmCPS1. SmCPS1 is a key enzyme on the biosynthesis pathway of tanshinone (a compound used to treat cardiovascular diseases). The tac promoter, a strong hybrid promoter, is used to control and increase the expression levels of the target gene (SmCPS1), and is used in the expression of recombinant proteins, produced from the combination of promoters from the trp and lac operons. The GST label is used to purify the recombinant protein. The GFP is used to measure the expression level of the part.	+	This part is a complete gene circuit that can be used to produce the enzyme SmCPS1. SmCPS1 is a key enzyme on the biosynthesis pathway of tanshinone (a compound used to treat cardiovascular diseases). The tac promoter, a strong hybrid promoter, is used to control and increase the expression levels of the target gene (SmCPS1), and is used in the expression of recombinant proteins, produced from the combination of promoters from the trp and lac operons. The GST label is used to purify the recombinant protein. The GFP is used to measure the expression level of the part.