Difference between revisions of "Part:BBa K1951006"
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| + | == General information about flagellin (FliC protein)== | ||
| + | To see an exemple of this biobrick functionality, you can visit our composite part : [https://parts.igem.org/Part:BBa_K1951009 BBa_K1951009] | ||
| − | + | [[File:T--Aix-Marseille--Pb_biosorption.png|10000px|left|thumb|Representative electron micrographs of nanoparticles produced by ''D. | |
| − | < | + | alaskensis'' G20. Pd is found on the surface of the cell <ref> https://www.ncbi.nlm.nih.gov/pubmed/25686718 </ref> ]] |
| − | This coding sequence codes flagellin C which is a main protein involved for the extracellular flagellar establishment. Flagellar enables bacterial swimming. It has been shown that the flagellin structure from Desulfovibrio | + | This coding sequence codes flagellin C which is a main protein involved for the extracellular flagellar establishment. Flagellar enables bacterial swimming. It has been shown that the flagellin structure from ''Desulfovibrio'' can adsorb metallic nanoparticules as gold or platinum. <ref> https://www.ncbi.nlm.nih.gov/pubmed/25686718 </ref> |
| − | + | == Design summary == | |
| − | === | + | Starting from the ''Desulfovibrio vulgaris'' strain Hildenborough flagellin sequence ( http://www.ncbi.nlm.nih.gov/nucleotide/46451220?report=genbank&log$=nucltop&blast_rank=3&RID=WDB55ADB014 ) we have designed a part to produce this protein in <i>E. coli</i> chassis [https://parts.igem.org/Part:BBa_K1951008 BBa_K1951009]. |
| + | All forbidden restriction sites have been removed. | ||
| + | Codons have been optimized for <i> E.coli </i> to allow a maximal transcription level. | ||
| − | + | == Proof of functionality == | |
| − | + | ||
| − | + | ||
| + | This sequence has been tested: placed under the control of a strong promoter with a ribosome binding site the sequence has been <u>validated as functional</u>. For further information, go on the following link : [https://parts.igem.org/Part:BBa_K1951009:Design BBa_K1951009 design] | ||
| + | |||
| + | ==Sequence and Features== | ||
| + | <partinfo>BBa_K1951005 SequenceAndFeatures</partinfo> | ||
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Latest revision as of 12:40, 17 October 2016
Contents
General information about flagellin (FliC protein)
To see an exemple of this biobrick functionality, you can visit our composite part : BBa_K1951009
This coding sequence codes flagellin C which is a main protein involved for the extracellular flagellar establishment. Flagellar enables bacterial swimming. It has been shown that the flagellin structure from Desulfovibrio can adsorb metallic nanoparticules as gold or platinum. [2]
Design summary
Starting from the Desulfovibrio vulgaris strain Hildenborough flagellin sequence ( http://www.ncbi.nlm.nih.gov/nucleotide/46451220?report=genbank&log$=nucltop&blast_rank=3&RID=WDB55ADB014 ) we have designed a part to produce this protein in E. coli chassis BBa_K1951009. All forbidden restriction sites have been removed. Codons have been optimized for E.coli to allow a maximal transcription level.
Proof of functionality
This sequence has been tested: placed under the control of a strong promoter with a ribosome binding site the sequence has been validated as functional. For further information, go on the following link : BBa_K1951009 design
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1224
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 301
Illegal AgeI site found at 709 - 1000COMPATIBLE WITH RFC[1000]