Difference between revisions of "Part:BBa K1951005"
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Flagellin is the main protein which polymerizes to form the filaments of bacterial flagella and is necessary for bacterial movement by swimming. The possession of a flagellum allow bacteria to swim towards food sources and so gives them a selective advantage. <ref> https://www.ncbi.nlm.nih.gov/gene/949101 </ref> | Flagellin is the main protein which polymerizes to form the filaments of bacterial flagella and is necessary for bacterial movement by swimming. The possession of a flagellum allow bacteria to swim towards food sources and so gives them a selective advantage. <ref> https://www.ncbi.nlm.nih.gov/gene/949101 </ref> | ||
| − | It has been demonstrated that | + | It has been demonstrated that flagellin has the ability to adsorb precious metals such as platinum or gold. |
== Design summary == | == Design summary == | ||
Starting from the <i>E.coli</i> flagellin sequence ( http://www.uniprot.org/uniprot/P04949 ) we have designed a part to produce this protein in <i>E. coli</i> chassis [https://parts.igem.org/Part:BBa_K1951008 BBa_K1951008]. | Starting from the <i>E.coli</i> flagellin sequence ( http://www.uniprot.org/uniprot/P04949 ) we have designed a part to produce this protein in <i>E. coli</i> chassis [https://parts.igem.org/Part:BBa_K1951008 BBa_K1951008]. | ||
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All forbidden restriction sites have been removed. | All forbidden restriction sites have been removed. | ||
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Codons have been optimized for <i> E.coli </i> to allow a maximal transcription level. | Codons have been optimized for <i> E.coli </i> to allow a maximal transcription level. | ||
Latest revision as of 12:38, 17 October 2016
Contents
General information about flagellin (FliC protein)
To see an exemple of this biobrick functionality, you can visit our composite part : BBa_K1951008
Flagellin is the main protein which polymerizes to form the filaments of bacterial flagella and is necessary for bacterial movement by swimming. The possession of a flagellum allow bacteria to swim towards food sources and so gives them a selective advantage. [1]
It has been demonstrated that flagellin has the ability to adsorb precious metals such as platinum or gold.
Design summary
Starting from the E.coli flagellin sequence ( http://www.uniprot.org/uniprot/P04949 ) we have designed a part to produce this protein in E. coli chassis BBa_K1951008.
All forbidden restriction sites have been removed.
Codons have been optimized for E.coli to allow a maximal transcription level.
Proof of functionality
This sequence has been tested: placed under the control of a strong promoter with a ribosome binding site the sequence has been validated as functional. For further information, go on the following link : BBa_K1951008 design
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1224
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 301
Illegal AgeI site found at 709 - 1000COMPATIBLE WITH RFC[1000]