Difference between revisions of "Part:BBa K1951005"

(→‎Proof of fonctionnality)
 
(15 intermediate revisions by 3 users not shown)
Line 1: Line 1:
 +
== General information about flagellin (FliC protein)==
 +
To see an exemple of this biobrick functionality, you can visit our composite part : [https://parts.igem.org/Part:BBa_K1951008 BBa_K1951008]
  
−
__NOTOC__
+
[[File:T--Aix-Marseille--flagellum.jpeg|600px|left]]
−
<partinfo>BBa_K1951005 short</partinfo>
+
  
−
This coding sequence codes the Flagellin C (FliC) protein, filament of the flagellum from <i>Escherichia coli</i> strain.
+
Flagellin is the main protein which polymerizes to form the filaments of bacterial flagella and is necessary for bacterial movement by swimming. The possession of a flagellum allow bacteria to swim towards food sources and so gives them a selective advantage. <ref> https://www.ncbi.nlm.nih.gov/gene/949101 </ref>
  
−
[[File:T--Aix-Marseille--flagellum.jpeg|600px|left]]
+
It has been demonstrated that flagellin has the ability to adsorb precious metals such as platinum or gold.
  
−
FliC is the main protein constitutive of the flagellum filament and is involved to promote bacterial swimming. In the possession of flagellum, bacteria can move what confers them a selective advantage.
+
== Design summary ==
−
It has been demonstrated that Flagellin has the ability to adsorbe precious metal such as Platinum, gold..  
+
Starting from the <i>E.coli</i> flagellin sequence ( http://www.uniprot.org/uniprot/P04949 ) we have designed a part to produce this protein in <i>E. coli</i> chassis [https://parts.igem.org/Part:BBa_K1951008 BBa_K1951008].
  
−
=== Design resum ===
+
All forbidden restriction sites have been removed.
−
Codons have been optimized for <i> Escherichia coli </i> allowing a high transcription level.  
+
  
−
Every forbidden restriction sites have been removed and sequence is optimital.
+
Codons have been optimized for <i> E.coli </i> to allow a maximal transcription level.
  
−
=== Proof of fonctionnality ===  
+
== Proof of functionality ==  
  
−
This sequence has been tested under the control of a strong promoter and <u>validated as fonctionnal</u>. For further information, go on the following link : [https://parts.igem.org/Part:BBa_K1951008:Design BBa_K1951008 design]
+
This sequence has been tested: placed under the control of a strong promoter with a ribosome binding site the sequence has been <u>validated as functional</u>. For further information, go on the following link : [https://parts.igem.org/Part:BBa_K1951008:Design BBa_K1951008 design]
 
    
 
    
−
<!-- Add more about the biology of this part here
+
==Sequence and Features==
−
===Usage and Biology===
+
−
 
+
−
<!-- -->
+
−
<span class='h3bb'>Sequence and Features</span>
+
 
<partinfo>BBa_K1951005 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1951005 SequenceAndFeatures</partinfo>
  

Latest revision as of 12:38, 17 October 2016

General information about flagellin (FliC protein)

To see an exemple of this biobrick functionality, you can visit our composite part : BBa_K1951008

T--Aix-Marseille--flagellum.jpeg

Flagellin is the main protein which polymerizes to form the filaments of bacterial flagella and is necessary for bacterial movement by swimming. The possession of a flagellum allow bacteria to swim towards food sources and so gives them a selective advantage. [1]

It has been demonstrated that flagellin has the ability to adsorb precious metals such as platinum or gold.

Design summary

Starting from the E.coli flagellin sequence ( http://www.uniprot.org/uniprot/P04949 ) we have designed a part to produce this protein in E. coli chassis BBa_K1951008.

All forbidden restriction sites have been removed.

Codons have been optimized for E.coli to allow a maximal transcription level.

Proof of functionality

This sequence has been tested: placed under the control of a strong promoter with a ribosome binding site the sequence has been validated as functional. For further information, go on the following link : BBa_K1951008 design

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1224
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 301
    Illegal AgeI site found at 709
  • 1000
    COMPATIBLE WITH RFC[1000]


  1. ↑ https://www.ncbi.nlm.nih.gov/gene/949101