Difference between revisions of "Part:BBa K1899006:Experience"
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| − | [[File: | + | [[File:IT--Hong_Kong_HKUST--Phlfp_C.jpg|thumb|600px|center|<b>Fig a) Comparison of Technical Triplicate Results of pSB3K3-BBa_J23101-B0032-C0012-B1006- PhlFp -E0240 and pSB3K3-PhlFp - BBa_E0240</b>The fold change between <b>pSB3K3-PhlFp - BBa_E0240 and negative control (pSB3K3-BBa_E0240)</b> is 13.2, which is same as that of <b>pSB3K3-BBa_J23101-B0032-C0012-B1006- PhlFp -E0240</b>. Since their RFU are similar and almost the same, it proves that there is no cross-talk between <i>lacp</i> and <i>Phlfp</i>(<bbpart>(BBa_K1899004<bbpart>). ]] |
| − | <b>Fig a) Comparison of Technical Triplicate Results of pSB3K3-BBa_J23101-B0032-C0012-B1006- PhlFp -E0240 and pSB3K3-PhlFp - BBa_E0240</b> | + | |
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| − | The fold change between <b>pSB3K3-PhlFp - BBa_E0240 and negative control (pSB3K3-BBa_E0240)</b> is 13.2, which is same as that of <b>pSB3K3-BBa_J23101-B0032-C0012-B1006- PhlFp -E0240</b>. Since their RFU are similar and almost the same, it proves that there is no cross-talk between <i>lacp</i> and <i>Phlfp</i>(<bbpart>(BBa_K1899004<bbpart>). ]] | + | |
| + | [[File:IGEM2016 HKUST constructA.png|thumb|600px|center|<b>Fig 1. Comparison on the strength of <i>phlFp</i>, <i>tetp</i> and <i>lacp</i>.</b> Negative control represents BBa_E0240. Characterization was done using <i>E. coli</i> strain JW0336. Cells were first precultured overnight and were subcultured to mid-log phase where GFP emission measurements were made using an EnVision® multilabel reader. This result was obtained by combining 3 characterization data obtained in 3 different days. Error bar present SD from 3 biological replicates.]] | ||
Revision as of 11:54, 17 October 2016
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Applications of BBa_K1899006
Characterisation Result
[[File:IT--Hong_Kong_HKUST--Phlfp_C.jpg|thumb|600px|center|Fig a) Comparison of Technical Triplicate Results of pSB3K3-BBa_J23101-B0032-C0012-B1006- PhlFp -E0240 and pSB3K3-PhlFp - BBa_E0240The fold change between pSB3K3-PhlFp - BBa_E0240 and negative control (pSB3K3-BBa_E0240) is 13.2, which is same as that of pSB3K3-BBa_J23101-B0032-C0012-B1006- PhlFp -E0240. Since their RFU are similar and almost the same, it proves that there is no cross-talk between lacp and Phlfp(BBa_K1899004