Difference between revisions of "Part:BBa K2065007:Experience"

Revision as of 11:52, 17 October 2016

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K2065007

iGEM TU Eindhoven 2016

iGEM TU Eindhoven used this part in combination with CT52-LargeBiT (BBa_K2065000). These two proteins could dimerize on our heterodimeric T-14-3-3 scaffold proteins with mutation which were designed by our team. When dimerized the functional luciferase protein was formed. This protein showed luminescence at 460 nm. Thus this system was used as a read out method for T14-3-3 heterodimers.

User Reviews

UNIQ718bd802e10b3dee-partinfo-00000000-QINU UNIQ718bd802e10b3dee-partinfo-00000001-QINU

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 iGEM TU Eindhoven 2016
-iGEM TU Eindhoven used this part in combination with CT52-LargeBiT (BBa_K2065007). These two proteins could dimerize on our heterodimeric T-14-3-3 scaffold proteins with mutation which were designed by our team. When dimerized the functional luciferase protein was formed. This protein showed luminescence at 460 nm. Thus this system was used as a read out method for T14-3-3 heterodimers.
+iGEM TU Eindhoven used this part in combination with CT52-LargeBiT (BBa_K2065000). These two proteins could dimerize on our heterodimeric T-14-3-3 scaffold proteins with mutation which were designed by our team. When dimerized the functional luciferase protein was formed. This protein showed luminescence at 460 nm. Thus this system was used as a read out method for T14-3-3 heterodimers.
 ===User Reviews===