Difference between revisions of "Part:BBa K1951005"
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| − | + | == General information about flagellin (FliC protein)== | |
| − | + | To see an exemple of this biobrick functionality, you can visit our composite part : [https://parts.igem.org/Part:BBa_K1951008 BBa_K1951008] | |
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[[File:T--Aix-Marseille--flagellum.jpeg|600px|left]] | [[File:T--Aix-Marseille--flagellum.jpeg|600px|left]] | ||
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It has been demonstrated that Flagellin has the ability to adsorb precious metals such as Platinum, Gold.. | It has been demonstrated that Flagellin has the ability to adsorb precious metals such as Platinum, Gold.. | ||
| − | + | == Design summary == | |
Starting from the <i>E.coli</i> flagellin sequence ( http://www.uniprot.org/uniprot/P04949 ) we have designed a part to produce this protein in <i>E. coli</i> chassis [https://parts.igem.org/Part:BBa_K1951008 BBa_K1951008]. | Starting from the <i>E.coli</i> flagellin sequence ( http://www.uniprot.org/uniprot/P04949 ) we have designed a part to produce this protein in <i>E. coli</i> chassis [https://parts.igem.org/Part:BBa_K1951008 BBa_K1951008]. | ||
All forbidden restriction sites have been removed. | All forbidden restriction sites have been removed. | ||
Codons have been optimized for <i> E.coli </i> to allow a maximal transcription level. | Codons have been optimized for <i> E.coli </i> to allow a maximal transcription level. | ||
| − | + | == Proof of functionality == | |
This sequence has been tested: placed under the control of a strong promoter with a ribosome binding site the sequence has been <u>validated as functional</u>. For further information, go on the following link : [https://parts.igem.org/Part:BBa_K1951008:Design BBa_K1951008 design] | This sequence has been tested: placed under the control of a strong promoter with a ribosome binding site the sequence has been <u>validated as functional</u>. For further information, go on the following link : [https://parts.igem.org/Part:BBa_K1951008:Design BBa_K1951008 design] | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
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==Sequence and Features== | ==Sequence and Features== | ||
Revision as of 11:07, 17 October 2016
Contents
General information about flagellin (FliC protein)
To see an exemple of this biobrick functionality, you can visit our composite part : BBa_K1951008
Flagellin is the main protein which polymerizes to form the filaments of bacterial flagella and is necessary for bacterial movement by swimming. The possession of a flagellum allow bacteria to swim towards food sources and so gives them a selective advantage. [1]
It has been demonstrated that Flagellin has the ability to adsorb precious metals such as Platinum, Gold..
Design summary
Starting from the E.coli flagellin sequence ( http://www.uniprot.org/uniprot/P04949 ) we have designed a part to produce this protein in E. coli chassis BBa_K1951008. All forbidden restriction sites have been removed. Codons have been optimized for E.coli to allow a maximal transcription level.
Proof of functionality
This sequence has been tested: placed under the control of a strong promoter with a ribosome binding site the sequence has been validated as functional. For further information, go on the following link : BBa_K1951008 design
Usage and Biology
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1224
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 301
Illegal AgeI site found at 709 - 1000COMPATIBLE WITH RFC[1000]