Difference between revisions of "Part:BBa K1930005:Design"
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===References=== | ===References=== | ||
− | <p>[https://www.ncbi.nlm.nih.gov/pubmed/25661487 | + | <p>[https://www.ncbi.nlm.nih.gov/pubmed/25661487 ''Sinai et al.''] </p> |
<p>[http://subtiwiki.uni-goettingen.de/bank/index.php?gene=atpA Subtiwiki-atpA]</p> | <p>[http://subtiwiki.uni-goettingen.de/bank/index.php?gene=atpA Subtiwiki-atpA]</p> |
Revision as of 10:48, 17 October 2016
PAtpI
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The promoter PAtpI is a constitutive promoter which has its origin in Bacillus subtilis. It is responsible for the expression of atpA gene (ATP synthesis) during the first 30 minutes of the germination of B. subtilis. atpA gene is part of an operon (atpI-atpB-atpE-atpF-atpH-atpA-atpG-atpD-atpC), therefore the promoter region in front of the first protein coding gene (atpI) in this operon was chosen.
The region was checked for the binding of sigma factors and transcription factors with DBTBS. No binding factors were found with the highest significance level. In our project we wanted to find a constitutive ON promoter for our ciprofloxacin resistance casette. In the following part we put the promoter PAtpI in the pSB1C3 backbone to make it available to other iGEM teams. For more information see plasmid construction under our lab journal: http://2016.igem.org/Team:Groningen/Experiments
Source
Ordered as gBlock. IDT (Integrated DNA technologies).
References
[http://subtiwiki.uni-goettingen.de/bank/index.php?gene=atpA Subtiwiki-atpA]