Difference between revisions of "Part:BBa K1983000"

 
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<partinfo>BBa_K1983000 short</partinfo>
 
<partinfo>BBa_K1983000 short</partinfo>
  
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This is an improved Stanford-Brown 2013 PAL biobrick part. We have removed the T7 promoter and replaced the N-terminal FLAG-tag with a more common C-terminal 6xHis-tag. The C-terminal tag location was chosen assuming it would less likely affect the stability and folding of the protein.
  
 
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Revision as of 09:19, 17 October 2016


Codon optimized AvPAL with C-terminal 6XHis-Tag

This is an improved Stanford-Brown 2013 PAL biobrick part. We have removed the T7 promoter and replaced the N-terminal FLAG-tag with a more common C-terminal 6xHis-tag. The C-terminal tag location was chosen assuming it would less likely affect the stability and folding of the protein.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1265
    Illegal XhoI site found at 1702
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1483
  • 1000
    COMPATIBLE WITH RFC[1000]