Difference between revisions of "Part:BBa K1959003"

Revision as of 08:49, 17 October 2016

BKT coding sequence fused with Pea transit peptide

This part is consist of a Pea transit peptide and the coding sequence of β-carotene ketolase originally derived from Chlamydomonas reinhardtii. β-carotene ketolase realizes the conversion of zeaxanthin to astaxanthin, the final step of astaxanthin biosynthesis. Sequence of β-carotene ketolase has been optimized for better expression in rice (Oryza sativa).

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 1085
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 478
Illegal SapI site found at 900

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K1959003 short</partinfo>
-This part is consist of a Pea transit peptide and the coding sequence of beta-carotene ketolase originally derived from
+This part is consist of a <em>Pea</em> transit peptide and the coding sequence of β-carotene ketolase originally derived from
-Chlamydomonas reinhardtii. Beta-carotene ketolase realizes the convertion of zeaxanthin to astaxanthin, the final step of astaxanthin biosynthesis. Sequence of beta-carotene ketolase has been optimized for better expression in Oryza sativa.
+<em>Chlamydomonas reinhardtii</em>. β-carotene ketolase realizes the conversion of zeaxanthin to astaxanthin, the final step of astaxanthin biosynthesis. Sequence of β-carotene ketolase has been optimized for better expression in rice (<em>Oryza sativa</em>).
 <!-- Add more about the biology of this part here