Difference between revisions of "Part:BBa K2136010"
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__NOTOC__
 
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<partinfo>BBa_K2136010 short</partinfo>
 
<partinfo>BBa_K2136010 short</partinfo>
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Synthethic cassette for algae expression.
 
Synthethic cassette for algae expression.
  

Revision as of 23:58, 16 October 2016


5' cassete for Chlamydomonas transgenic expression

Synthethic cassette for algae expression.

This composite part is the 5' part of a system to express proteins in Chlamydomonas reinhardtii. It includes a promoter, a resistance gene, a self cleaving peptide and a signal peptide. This combination, in conjunction with a in-frame coding sequence and a microalgae terminator enables the efficient translation and secretion to the growing media of the constructs of interest.

This part was characterized through the expression of a codon optimized version of mCherry reporter protein (https://parts.igem.org/Part:BBa_K2136016).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 264
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1539
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1283
    Illegal NgoMIV site found at 1344
  • 1000
    COMPATIBLE WITH RFC[1000]