Difference between revisions of "Part:BBa K2136018"
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This sequence is part of the arylsuflatase 2 (Ars2) gene in Chlamydomonas reinhardtii. It is responsible for allowing the secretion of the fused protein to the culture medium, thus allowing cell-lysys independent purification of proteins and/or secretion for other aplications | This sequence is part of the arylsuflatase 2 (Ars2) gene in Chlamydomonas reinhardtii. It is responsible for allowing the secretion of the fused protein to the culture medium, thus allowing cell-lysys independent purification of proteins and/or secretion for other aplications | ||
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| + | This part also includes a 3' scar with XhoI and ClaI restriction sites included in original gene synthesis to better suit team USP_UNIFESP 2016's workflow. | ||
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Latest revision as of 23:42, 16 October 2016
Ars2 signal peptide for secretion in Chlamydomonas
This sequence is part of the arylsuflatase 2 (Ars2) gene in Chlamydomonas reinhardtii. It is responsible for allowing the secretion of the fused protein to the culture medium, thus allowing cell-lysys independent purification of proteins and/or secretion for other aplications
This part also includes a 3' scar with XhoI and ClaI restriction sites included in original gene synthesis to better suit team USP_UNIFESP 2016's workflow.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 85
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]