Difference between revisions of "Part:BBa K1930004:Design"
Barawaclawik (Talk | contribs) (→References) |
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===References=== | ===References=== | ||
− | [https://www.ncbi.nlm.nih.gov/gene/20467117 ''Gene ID''] | + | <p>[https://www.ncbi.nlm.nih.gov/gene/20467117 ''Gene ID'']</p> |
− | [https://www.ncbi.nlm.nih.gov/pubmed/19822894 ''Quinolone resistance''] | + | <p>[https://www.ncbi.nlm.nih.gov/pubmed/19822894 ''Quinolone resistance'']</p> |
Revision as of 23:40, 16 October 2016
Ciprofloxacin resistance cassette
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Unknown
- 12INCOMPATIBLE WITH RFC[12]Unknown
- 21INCOMPATIBLE WITH RFC[21]Unknown
- 23INCOMPATIBLE WITH RFC[23]Unknown
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
To design a ciprofloxacin resistance cassette we designed a gBlock that contains the Bacillus subtilis promoter PAtpI, which is active from a very early stage of germination and includes a ribosome binding site. The gBlock also contains the original qnrS1 gene sequence from E. coli, the double terminator BBa_B0015 from iGEM as well as the prefix and suffix for BioBricks. In summary the ciprofloxacin cassette consists of the following parts PAtpI+RBS+qnrS1+2TER.
Source
Ordered as gBlock. IDT (Integrated DNA technologies).