Difference between revisions of "Part:BBa K1930004:Design"
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===References=== | ===References=== | ||
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+ | [https://www.ncbi.nlm.nih.gov/gene/20467117 ''Gene ID''] | ||
+ | [https://www.ncbi.nlm.nih.gov/pubmed/19822894 ''Quinolone resistance''] |
Revision as of 23:39, 16 October 2016
Ciprofloxacin resistance cassette
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 536
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
To design a ciprofloxacin resistance cassette we designed a gBlock that contains the Bacillus subtilis promoter PAtpI, which is active from a very early stage of germination and includes a ribosome binding site. The gBlock also contains the original qnrS1 gene sequence from E. coli, the double terminator BBa_B0015 from iGEM as well as the prefix and suffix for BioBricks. In summary the ciprofloxacin cassette consists of the following parts PAtpI+RBS+qnrS1+2TER.
Source
Ordered as gBlock. IDT (Integrated DNA technologies).