Difference between revisions of "Part:BBa K1936001:Design"
Mdickmanns (Talk | contribs) (→Source) |
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===Source=== | ===Source=== | ||
− | + | All used sequences except for linkers where codon optimized for use in H.sapiens. Therefore base sequences may not match with mentioned sources. | |
+ | <html><br></html> | ||
Tom5 yeast sequence:<html><br></html> | Tom5 yeast sequence:<html><br></html> | ||
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LOV2 sequence:<html><br></html> | LOV2 sequence:<html><br></html> | ||
from pKM291 containing LOVpep[T406A,T407A] double mutant [1] | from pKM291 containing LOVpep[T406A,T407A] double mutant [1] | ||
− | <br> | + | <html><br></html> |
===References=== | ===References=== | ||
<html><font size="-1">[1]</font><font size="-2"> Müller K, Engesser R, Timmer J, Zurbriggen MD, Weber W (2014) Orthogonal optogenetic triple-gene control in Mammalian cells. ACS Synth Biol. 2014 Nov 21;3(11):796-801. doi: 10.1021/sb500305v. Epub 2014 Oct 28.</font></html> | <html><font size="-1">[1]</font><font size="-2"> Müller K, Engesser R, Timmer J, Zurbriggen MD, Weber W (2014) Orthogonal optogenetic triple-gene control in Mammalian cells. ACS Synth Biol. 2014 Nov 21;3(11):796-801. doi: 10.1021/sb500305v. Epub 2014 Oct 28.</font></html> |
Revision as of 21:21, 16 October 2016
Tom5-eGFP-LOV2
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Unknown
- 21INCOMPATIBLE WITH RFC[21]Unknown
- 23INCOMPATIBLE WITH RFC[23]Unknown
- 25INCOMPATIBLE WITH RFC[25]Unknown
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1226
Design Notes
ALERT !!!Under construction !!
Source
All used sequences except for linkers where codon optimized for use in H.sapiens. Therefore base sequences may not match with mentioned sources.
Tom5 yeast sequence:
http://www.ebi.ac.uk/ena/data/view/AY558369
LOV2 sequence:
from pKM291 containing LOVpep[T406A,T407A] double mutant [1]
References
[1] Müller K, Engesser R, Timmer J, Zurbriggen MD, Weber W (2014) Orthogonal optogenetic triple-gene control in Mammalian cells. ACS Synth Biol. 2014 Nov 21;3(11):796-801. doi: 10.1021/sb500305v. Epub 2014 Oct 28.