Difference between revisions of "Part:BBa K1936001:Design"

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LOV2 sequence:<html><br></html>
 
LOV2 sequence:<html><br></html>
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from pKM291 (double mutant)<html><br></html>
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from pKM291 containing LOVpep[T406A,T407A] double mutant [1]
  
 
===References===
 
===References===

Revision as of 21:09, 16 October 2016


Tom5-eGFP-LOV2


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 33
    Illegal BamHI site found at 712
    Illegal XhoI site found at 515
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1226


Design Notes

ALERT !!!Under construction !!


Source

Note: All used sequences except for linkers where codon optimized for use in H.sapiens. Therefore base sequences may not match with mentioned sources.


Tom5 yeast sequence:
http://www.ebi.ac.uk/ena/data/view/AY558369

eGFP sequence:

LOV2 sequence:
from pKM291 containing LOVpep[T406A,T407A] double mutant [1]

References