Difference between revisions of "Part:BBa K1936001:Design"

(→‎Source)
(→‎Source)
Line 15: Line 15:
  
  
−
Tom5 yeast sequence:
+
Tom5 yeast sequence:<html><br></html>
 
http://www.ebi.ac.uk/ena/data/view/AY558369
 
http://www.ebi.ac.uk/ena/data/view/AY558369
  
 
eGFP sequence:
 
eGFP sequence:
  
−
LOV2 sequence:
+
LOV2 sequence:<html><br></html>
−
(double mutant)
+
from pKM291 (double mutant)<html><br></html>
  
 
===References===
 
===References===

Revision as of 20:53, 16 October 2016


Tom5-eGFP-LOV2


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 33
    Illegal BamHI site found at 712
    Illegal XhoI site found at 515
  • 23
    INCOMPATIBLE WITH RFC[23]
    Unknown
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1226


Design Notes

ALERT !!!Under construction !!


Source

Note: All used sequences except for linkers where codon optimized for use in H.sapiens. Therefore base sequences may not match with mentioned sources.


Tom5 yeast sequence:
http://www.ebi.ac.uk/ena/data/view/AY558369

eGFP sequence:

LOV2 sequence:
from pKM291 (double mutant)

References