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| | <partinfo>BBa_K2170002 parameters</partinfo> | | <partinfo>BBa_K2170002 parameters</partinfo> |
| | <!-- --> | | <!-- --> |
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| − | ===Design Notes===
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| − |
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| − | '''Related BioBrick:'''
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| − | <!--*Other versions:[https://parts.igem.org/wiki/index.php?title=Part:BBa_?????? BBa_??????: Name_of_part] -->
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| − | <!--*Related BioBricks:[https://parts.igem.org/wiki/index.php?title=Part:BBa_?????? BBa_??????: Name_of_part]
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| − |
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| − | '''Cloning details:'''<br>
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| − | <!--*Designed in RFC10/RFC23/RFC25-->
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| − | <!--*Mutation C889G to delete XbaI restriction site-->
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| − | <!--*Truncation upstream/downstream compared to template, ?explanation?-->
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| − |
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| − | '''Quality control measures:'''<br>
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| − | <!--*Test digestion using ?enzyme1? & ?enzyme2?/Not yet performed-->
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| − | <!--*Sequencing using primer ?primer_name?/Not yet sequenced-->
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| − | <!--*Part was partly sequenced/Part was totally sequenced-->
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| − | '''Backbone:'''<br>
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| − | <!--*Backbone name: pSB1C3'/?backbone_name?-->
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| − | <!--*Resistance: Amp/Cp/Kan/-->
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| − | <!--*Copynumber: low/medium/high-->
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| − |
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| − | '''Protein coding:'''<br>
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| − | <!--*Protein: ?Name_of_gene_product? [Nucleotide 1 to ???]-->
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| − | <!--*The protein has the amino acid replacements ???99??? to ???99???.-->
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| − | <!--*The protein encoded is posttranslationally modified by ???.-->
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| − | <!--*Tag: n-terminally fused/c-terminally fused His5/His6/Strep/Flag/other-->
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| − |
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| − | '''Enzymatic activity:'''
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| − | <!--none/EC-number ?.?.?.?-->
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| − |
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| − | '''Cytotoxicity:'''<br>
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| − | <!--none/not known/cytotoxic for ''organism name''-->
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| − |
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| − | '''Safety notes:'''<br>
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| − | <!--Known and anticipated sefety issues: none/health_risk/environmental_risk/other_risk-->
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| − | <!--Known and anticipated security issues: none/other.-->
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| − |
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| − | '''Intellectual property:'''
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| − | <!--Information on patent situation.-->
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| − | <!--Intellectual property claims made by the authors.-->
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| − |
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| − | '''Corresponding part author/authors:'''
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| − | <!--https://igem.org/User_Information.cgi?user_id=????/email-->
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| − |
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| − | ===Source===
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| − |
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| − | '''Source:'''<br>
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| − | <!--*Commercial system: plasmid name, system name, company name-->
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| − | <!--*Plasmid: p???, provided by ?name_of_person?, ?institute/university?, ?country?-->
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| − | <!--*Preexisting BioBrick ?Bba_number?-->
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| − | <!--*cDNA Clone: ?clone_name?, ?company_name?-->
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| − | <!--*Synthesized by ?company_name?.-->
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| − |
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| − | <!--'''Forward Primer:'''<br><code>5'- ??? - 3'</code><br>-->
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| − | <!--'''Reverse Primer:'''<br><code>5'- ??? - 3'</code><br>-->
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| − |
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| − | '''Organism:'''<br>
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| − | Genesequence derived from ''Escherichia coli''
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| − | <!--*Codonoptimized for ''?organism_name?''-->
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| − | <!--*Designed for the following Chassis: ''?organism-name?''-->
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| − | <!--*Statement about functionality in other chassis.-->
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| − |
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| − | <!--Thank you very much for using BBF RFC 82 to describe the present BioBrick. If you have any remarks or recommendations concerning this RFC we would highly appreciate to get your feedback on: []-->
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| − |
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| − | ===References===
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| − | <!-- Here you find templates to insert references to literature and different databases-->
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| − |
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| − | '''Literature references:'''<br>
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| − | <!--*[http://www.ncbi.nlm.nih.gov/pubmed/?PMID? '''Pubmed:''' ?Author(s)?, ?year?: ?title?]-->
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| − |
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| − | '''Database references:'''<br>
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| − | <!--*[http://www.ncbi.nlm.nih.gov/nuccore/?accessNr? '''GenBank''': ?title?]-->
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| − | <!--*[http://www.ebi.ac.uk/interpro/IEntry?ac=?accessNr? '''Interpro''': ?title?]-->
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| − | <!--*[http://www.uniprot.org/uniprot/?accessNr? '''Uniprot''': ?title?]-->
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| − | <!--*[http://pfam.sanger.ac.uk/family/?accessNr? '''Pfam:''' ?title?]-->
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| − | <!--*[http://www.rcsb.org/pdb/explore/explore.do?structureId=?accessNR? '''PDB:''' ?tile?]-->
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| − | <!--*[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=?accessNr? '''Branda:''' ?title?]-->
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Secretory eukaryotic biotin binding receptor with single chain avidin (CMV_BM40_scAvidin_A3C5_EGFR TMD_mRuby_StrepTag_polyA)
