Difference between revisions of "Part:BBa K2100003"
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<partinfo>BBa_K2100003 short</partinfo> | <partinfo>BBa_K2100003 short</partinfo> | ||
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+ | The pENTR hEF1a part is an entry vector containing the human elongation factor 1 alpha (hEF1a) promoter, a constituitive mammalian promoter. | ||
− | + | We have characterized the hEF1a promoter in all four cell lines we experimented on in the duration of our project using the following fluorescent tags: eYFP, mKate, and tagBFP. | |
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− | We have characterized the hEF1a promoter in all four cell lines we experimented on in the duration of our project using the following fluorescent tags: eYFP, mKate, and | + | |
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
<center><img src= "https://static.igem.org/mediawiki/parts/a/ae/T--MIT--attractenehef1aeyfp.png" alt = '' style="width:800px;height:412px;" margin: 0 1.5%; class="rotate90"></center> | <center><img src= "https://static.igem.org/mediawiki/parts/a/ae/T--MIT--attractenehef1aeyfp.png" alt = '' style="width:800px;height:412px;" margin: 0 1.5%; class="rotate90"></center> | ||
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+ | Our team used the entry vector of hEF1a to create an expression vector with many constructs. We often used | ||
Revision as of 17:13, 16 October 2016
pENTR hEF1a
The pENTR hEF1a part is an entry vector containing the human elongation factor 1 alpha (hEF1a) promoter, a constituitive mammalian promoter.
We have characterized the hEF1a promoter in all four cell lines we experimented on in the duration of our project using the following fluorescent tags: eYFP, mKate, and tagBFP.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 6
Illegal EcoRI site found at 1210
Illegal PstI site found at 337
Illegal PstI site found at 842 - 12INCOMPATIBLE WITH RFC[12]Unknown
- 21INCOMPATIBLE WITH RFC[21]Unknown
- 23INCOMPATIBLE WITH RFC[23]Unknown
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 6
Illegal EcoRI site found at 1210
Illegal PstI site found at 337
Illegal PstI site found at 842
Illegal NgoMIV site found at 725
Illegal AgeI site found at 103 - 1000COMPATIBLE WITH RFC[1000]